The lab says fixation will cause the dyes to leak with the cells. I do think the idea is the fact that fixation may possibly harm mobile membranes. I did find a paper which describes fixation applying methanol or acetic acid, following staining by using a DCFDA spinoff. DCFDA is https://2-fdck-hcl96925.acidblog.net/59155535/the-single-best-strategy-to-use-for-2-fdck-hcl