Mature sperm from Culex pipiens were isolated and analyzed by mass spectrometry to generate a mature sperm proteome dataset. In this study. we highlight subsets of proteins related to flagellar structure and sperm motility and compare the identified protein components to previous studies examining essential functions of sperm. The proteome includes 1700 unique protein IDs. https://www.alarecre.com/Wizarding-World-Harry-Potter-Scar-and-Glasses-iPhone-13-Pro-MagSafe-Case-p90262/